1M4Y

Crystal structure of HslV from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-15
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	I 2 2 2
Unit cell lengths	77.533, 93.748, 146.167
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.000 * - 2.100
Rwork	0.192
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.240 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.000 *	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.052 *	0.249 *
Total number of observations	334480 *
Number of reflections	31188
Completeness [%]	99.3	99.7
Redundancy	10.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	293	MPD, magnesium acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	reservoir	MPD	27.5 (%(w/v))
3	1	reservoir	magnesium acetate	200 (mM)
4	1	reservoir	sodium cacodylate	100 (mM)	pH6.5