1M4M

Mouse Survivin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-20
Detector	MAR CCD 165 mm
Wavelength(s)	1.2822, 1.2831, 1.2716
Spacegroup name	P 61 2 2
Unit cell lengths	41.430, 41.430, 291.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 * - 2.800
Rwork	0.243
R-free	0.31200
Structure solution method	AB INITIO PHASING
RMSD bond length	0.011
RMSD bond angle	1.540
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800 *	2.700
Rmerge	0.062 *	0.135 *
Total number of observations	23422 *
Number of reflections	4579 *
Completeness [%]	94.1 *	85.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	Isopropanol, cacodylate potassium chloride, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	Isopropanol, cacodylate potassium chloride, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	Isopropanol, cacodylate potassium chloride, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	isopropanol	10-20 (%)
2	1	reservoir	sodium cacodylate	0.05 (M)	pH6.0
3	1	reservoir		0.1 (M)
4	1	reservoir		0.05 (M)
5	1	drop	HEPES	10 (mM)	pH7.5
6	1	drop		150 (mM)
7	1	drop	BME	2 (mM)