1M4K
Crystal structure of the human natural killer cell activator receptor KIR2DS2 (CD158j)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.95370 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 97.535, 97.535, 54.375 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.000 * - 2.300 |
R-factor | 0.23 |
Rwork | 0.221 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | KIR2dL2 KIR2DL1 KIR2DL3 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.300 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.480 * | |
Number of reflections | 17624 | |
<I/σ(I)> | 4.6 | 2.6 |
Completeness [%] | 61.0 * | 99.6 |
Redundancy | 5.1 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | Ammonium sulfate, sodium acetate, Zinc chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13 (mg/ml) | |
2 | 1 | drop | Tris | 50 (mM) | pH8. |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | reservoir | ammonium sulfate | 1.3-1.6 (M) | |
5 | 1 | reservoir | sodium acetate | 50 (mM) | pH4.6 |
6 | 1 | reservoir | 20 (mM) |