1M4J
CRYSTAL STRUCTURE OF THE N-TERMINAL ADF-H DOMAIN OF MOUSE TWINFILIN ISOFORM-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8459 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.544, 74.488, 76.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.2354 |
| Rwork | 0.232 |
| R-free | 0.25100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.044 | 0.255 * |
| Total number of observations | 457961 * | |
| Number of reflections | 34257 * | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 93.0 * | 92.2 * |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | 1.6M Na-Citrate, 3% PEG 400, 0.1M Na-Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | Tris | 10 (mM) | pH7.5 |
| 4 | 1 | reservoir | sodium citrate | 1.6 (M) | |
| 5 | 1 | reservoir | PEG400 | 3 (%) | |
| 6 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
