1M46

CRYSTAL STRUCTURE OF MLC1P BOUND TO IQ4 OF MYO2P, A CLASS V MYOSIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-07-01
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	48.504, 121.504, 29.082
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.100 *
R-factor	0.2013
Rwork	0.199
R-free	0.23900 *
Structure solution method	Molecular replacement, Based on another structure of a mutant of MLC1P bound to IQ4 that crystallized in a different space group and was determined in the lab by the se-met mad method
RMSD bond length	0.022
RMSD bond angle	1.817
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.180
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.075	0.360
Number of reflections	10540
<I/σ(I)>	15	4.2
Completeness [%]	99.2 *	98.5
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4 *	293	ammonium sulfate, potassium/sodium tartrate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	2.2 (M)
2	1	1	potassium sodium tartrate tetrahydrate	0.2 (M)
3	1	1	trisodium citrate dihydrate	0.1 (M)	pH5.6
4	1	2	sodium acetate	5 (mM)	pH4.0
5	1	2		1 (mM)
6	1	2	dithiothreitol	1 (mM)
7	1	2	protein	10 (mM)