1M46
CRYSTAL STRUCTURE OF MLC1P BOUND TO IQ4 OF MYO2P, A CLASS V MYOSIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2000-07-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 48.504, 121.504, 29.082 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.100 * |
R-factor | 0.2013 |
Rwork | 0.199 |
R-free | 0.23900 * |
Structure solution method | Molecular replacement, Based on another structure of a mutant of MLC1P bound to IQ4 that crystallized in a different space group and was determined in the lab by the se-met mad method |
RMSD bond length | 0.022 |
RMSD bond angle | 1.817 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.180 |
High resolution limit [Å] | 2.100 * | 2.100 * |
Rmerge | 0.075 | 0.360 |
Number of reflections | 10540 | |
<I/σ(I)> | 15 | 4.2 |
Completeness [%] | 99.2 * | 98.5 |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4 * | 293 | ammonium sulfate, potassium/sodium tartrate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | ammonium sulfate | 2.2 (M) | |
2 | 1 | 1 | potassium sodium tartrate tetrahydrate | 0.2 (M) | |
3 | 1 | 1 | trisodium citrate dihydrate | 0.1 (M) | pH5.6 |
4 | 1 | 2 | sodium acetate | 5 (mM) | pH4.0 |
5 | 1 | 2 | 1 (mM) | ||
6 | 1 | 2 | dithiothreitol | 1 (mM) | |
7 | 1 | 2 | protein | 10 (mM) |