1M3S
Crystal structure of YckF from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-03-17 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9795, 0.9793, 0.9537 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 72.080, 72.080, 245.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.000 * - 1.950 |
| R-factor | 0.18897 |
| Rwork | 0.187 |
| R-free | 0.22400 * |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.814 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.010 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 28572 | |
| <I/σ(I)> | 31.6 | 5 |
| Completeness [%] | 99.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 8000, Na cacodylate, Ca acetate, Glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 8000, Na cacodylate, Ca acetate, Glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 14 (%) | |
| 3 | 1 | reservoir | sodium cacodylate | 80 (mM) | pH6.5 |
| 4 | 1 | reservoir | calcium acetate | 160 (mM) | |
| 5 | 1 | reservoir | glycerol | 20 (%) |
