1M38
Structure of Inorganic Pyrophosphatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Wavelength(s) | 0.91 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.690, 93.230, 69.840 |
Unit cell angles | 90.00, 99.74, 90.00 |
Refinement procedure
Resolution | 17.590 - 1.800 |
R-factor | 0.1932 |
Rwork | 0.193 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 2.561 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | BLANC |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 17.600 |
High resolution limit [Å] | 1.770 |
Rmerge | 0.037 |
Number of reflections | 63474 |
Completeness [%] | 99.9 |
Redundancy | 4.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | mes-buffer, cobalt chloride, imidodiphosphate, 2-methyl-2,4-pentadiole, pH 6.5, VAPOR DIFFUSION, temperature 298.0K |