1M2I
Crystal structure of E44A/E56A mutant of cytochrome b5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.693, 40.490, 39.199 |
| Unit cell angles | 90.00, 111.43, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.800 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.23200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1m20 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.045 | 0.228 |
| Number of reflections | 8616 | |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 88.9 | 56.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PHOSPHATE BUFFER, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PHOSPHATE BUFFER, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
