1M2H

Sir2 homologue S24A mutant-ADP ribose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	90
Detector technology	IMAGE PLATE
Detector	MAC Science DIP-2030
Spacegroup name	P 1 21 1
Unit cell lengths	35.707, 85.299, 44.853
Unit cell angles	90.00, 112.08, 90.00

Refinement procedure

Resolution	40.000 * - 1.700 *
R-factor	0.187
Rwork	0.187
R-free	0.21830 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.220 *
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	1.700 *
Rmerge	0.038 *
Total number of observations	70651 *
Number of reflections	24483 *
Completeness [%]	89.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mM)
2	1	reservoir	sodium citrate	100 (mM)	pH6.0
3	1	reservoir	PEG4000	14 (%)
4	1	reservoir	isopropanol	20 (%)