1M26
Crystal structure of jacalin-T-antigen complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.920, 78.000, 67.910 |
| Unit cell angles | 90.00, 100.73, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.620 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.20500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jac |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.800 * |
| Data reduction software | DENZO |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.580 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.073 | 0.289 * |
| Total number of observations | 188335 * | |
| Number of reflections | 74262 | 3714 * |
| Completeness [%] | 96.7 * | 95.8 |
| Redundancy | 2.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.3 | 298 | PEG 4000, 0.02M phosphate buffer at pH 7.3, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | phosphate | 0.02 (M) | pH7.3 |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | sodium azide | 0.025 (%(w/v)) | |
| 5 | 1 | drop | PEG4000 | 8-10 (%(w/v)) | |
| 6 | 1 | reservoir | PEG4000 | 40 (%) |
