1M20

Crystal Structure of F35Y Mutant of Trypsin-solubilized Fragment of Cytochrome b5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	70.709, 40.388, 39.299
Unit cell angles	90.00, 111.72, 90.00

Refinement procedure

Resolution	19.910 - 1.800
R-factor	0.193
Rwork	0.193
R-free	0.23800 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1es1
RMSD bond length	0.010
RMSD bond angle	1.080 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.910	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.061 *	0.315 *
Number of reflections	9129 *
Completeness [%]	94.3 *	79.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	phosphate buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	phosphate	3.1-3.2 (M)	pH7.5