1M1G
Crystal Structure of Aquifex aeolicus N-utilization substance G (NusG), Space Group P2(1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.500, 54.400, 113.200 |
Unit cell angles | 90.00, 89.30, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.251 |
Rwork | 0.251 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.510 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 * | 0.406 * |
Number of reflections | 69671 | |
<I/σ(I)> | 8.9 | 1.6 |
Completeness [%] | 94.9 | 79.2 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 18 * | PEG 3000, sodium chloride, pH 8.2, Vapor diffusion, sitting drop, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MES/Tris | 0.1 (M) | pH8.2 |
2 | 1 | reservoir | PEG3000 | 15 (%) | |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | drop | Tris-HCl | 20 (mM) | pH7.0 |
5 | 1 | drop | 50 (mM) | ||
6 | 1 | drop | DNA | 1 (M) |