1M1G

Crystal Structure of Aquifex aeolicus N-utilization substance G (NusG), Space Group P2(1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	87.500, 54.400, 113.200
Unit cell angles	90.00, 89.30, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.251
Rwork	0.251
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008 *
RMSD bond angle	1.510
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.080 *	0.406 *
Number of reflections	69671
<I/σ(I)>	8.9	1.6
Completeness [%]	94.9	79.2
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	18 *	PEG 3000, sodium chloride, pH 8.2, Vapor diffusion, sitting drop, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MES/Tris	0.1 (M)	pH8.2
2	1	reservoir	PEG3000	15 (%)
3	1	reservoir		0.2 (M)
4	1	drop	Tris-HCl	20 (mM)	pH7.0
5	1	drop		50 (mM)
6	1	drop	DNA	1 (M)