1M1D
TETRAHYMENA GCN5 WITH BOUND BISUBSTRATE ANALOG INHIBITOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2001-02-22 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0082 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.420, 67.830, 74.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.300 * - 2.200 |
| R-factor | 0.26 |
| Rwork | 0.213 |
| R-free | 0.26520 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qsn |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.750 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 * | 2.280 |
| High resolution limit [Å] | 2.200 * | 2.200 |
| Rmerge | 0.059 * | 0.346 * |
| Number of reflections | 26519 | |
| Completeness [%] | 99.2 * | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 20 * | Ammonium sulfate, sodium cacodylate, sodium chloride, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.36 (mM) | |
| 2 | 1 | drop | inhibitor | 0.58 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.6 |
| 5 | 1 | reservoir | 0.2 (M) |
