1M14
Tyrosine Kinase Domain from Epidermal Growth Factor Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-20 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.008 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 146.800, 146.800, 146.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.2397 |
| Rwork | 0.238 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1FGK; poly alanine |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.700 * |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (98.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.108 | 0.964 |
| Total number of observations | 248145 * | |
| Number of reflections | 16183 | 1568 * |
| <I/σ(I)> | 34 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.3 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium potassium phosphate | 1.0 (M) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | pH7.0 |
| 3 | 1 | drop | protein | 8 (mg/ml) |
