1M14

Tyrosine Kinase Domain from Epidermal Growth Factor Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-20
Detector	CUSTOM-MADE
Wavelength(s)	1.008
Spacegroup name	I 2 3
Unit cell lengths	146.800, 146.800, 146.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.2397
Rwork	0.238
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1FGK; poly alanine
RMSD bond length	0.010
RMSD bond angle	23.700 *
Data reduction software	TRUNCATE
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	X-PLOR (98.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.108	0.964
Total number of observations	248145 *
Number of reflections	16183	1568 *
<I/σ(I)>	34	1.8
Completeness [%]	100.0	100
Redundancy	15.3	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium potassium phosphate	1.0 (M)
2	1	reservoir	MES	0.1 (M)	pH7.0
3	1	drop	protein	8 (mg/ml)