1M0Z

Crystal Structure of the von Willebrand Factor Binding Domain of Glycoprotein Ib alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	C 1 2 1
Unit cell lengths	121.500, 54.500, 101.800
Unit cell angles	90.00, 103.70, 90.00

Refinement procedure

Resolution	40.000 * - 1.850
R-factor	0.189
Rwork	0.186
R-free	0.21700 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	26.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.073	0.337
Number of reflections	53895
<I/σ(I)>	16.3	2.9
Completeness [%]	97.8	80.8
Redundancy	3.6	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8 *	28 *	ammonium sulfate, lithium sulfate, CAPS, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 301K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	25 (mM)
2	1	drop		100 (mM)	pH7.8
3	1	drop	protein	4 (mg/ml)
4	1	reservoir	ammonium sulfate	1.8 (M)
5	1	reservoir	lithium sulfate	0.2 (M)
6	1	reservoir	CAPS	100 (mM)	pH8.2