1M0M
BACTERIORHODOPSIN M1 INTERMEDIATE AT 1.43 A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-08-20 |
Detector | ADSC QUANTUM |
Wavelength(s) | 0.979 |
Spacegroup name | P 63 |
Unit cell lengths | 61.069, 61.069, 110.099 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 1.430 |
R-factor | 0.1631 |
Rwork | 0.163 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3w |
RMSD bond length | 0.014 |
RMSD bond angle | 0.028 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.470 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.050 | 0.665 |
Total number of observations | 517904 * | |
Number of reflections | 39522 | |
<I/σ(I)> | 39.6 | 3.5 |
Completeness [%] | 92.2 | 96.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | CUBIC LIPID PHASE | 5.6 | 20 * | Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | sodium potassium Pi | 3.3 (M) | |
2 | 1 | 1 | protein | 3.5 (mg/ml) | |
3 | 1 | 1 | methylpentandiol | 0.05 (%) | |
4 | 1 | 1 | beta-octylglycopyranoside | 1.2 (%) |