1M07
RESIDUES INVOLVED IN THE CATALYSIS AND BASE SPECIFICITY OF CYTOTOXIC RIBONUCLEASE FROM BULLFROG (RANA CATESBEIANA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2000-08-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.629, 53.566, 72.203 |
| Unit cell angles | 90.00, 98.31, 90.00 |
Refinement procedure
| Resolution | 19.680 - 1.800 |
| R-factor | 0.227 |
| Rwork | 0.189 |
| R-free | 0.22900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1km8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 26.400 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.680 | 1.830 |
| High resolution limit [Å] | 1.800 * | 1.800 |
| Rmerge | 0.073 | 0.180 |
| Total number of observations | 137742 * | |
| Number of reflections | 20226 * | |
| <I/σ(I)> | 11.1 | 6.2 |
| Completeness [%] | 90.1 | 90.5 |
| Redundancy | 6.81 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | 0.1 M potassium sodium tartrate tetrahydrate, 24 % PEG 8000, 0.05 M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 4 (mM) | pH8.0 |
| 3 | 1 | drop | 4 (mM) | ||
| 4 | 1 | reservoir | potassium/sodium tartrate tetrahydrate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG8000 | 24 (%(w/v)) | |
| 6 | 1 | reservoir | sodium citrate | 0.05 (M) | pH5.6 |
