1M05

HLA B8 in complex with an Epstein Barr Virus determinant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Collection date	2002-03-15
Spacegroup name	P 21 21 21
Unit cell lengths	85.400, 90.100, 125.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.900
Rwork	0.242
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1agb
RMSD bond length	0.009 *
RMSD bond angle	1.380 *
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.900
Rmerge	0.083 *	0.306 *
Total number of observations	410938 *
Number of reflections	76165
Completeness [%]	99.1 *	100 *
Redundancy	5.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6 *	4 *	VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	10-15 (%)
3	1	reservoir	sodium citrate	0.1 (M)	pH5.6
4	1	reservoir	ammonium acetate	0.2 (M)
5	1	reservoir		10 (mM)