1LZW
Structural basis of ClpS-mediated switch in ClpA substrate recognition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 90.880, 114.590, 38.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 * - 2.500 |
R-factor | 0.243 |
Rwork | 0.243 |
R-free | 0.26900 * |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 20.900 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 * | |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.110 * | 0.478 * |
Total number of observations | 55297 * | |
Number of reflections | 26527 * | |
<I/σ(I)> | 17.9 | |
Completeness [%] | 98.3 * | 95.7 * |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 278 * | Zeth, K., (2002) Acta Crystallogr., D58, 1207. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 20 (mM) | ||
2 | 1 | drop | 20 (mM) | ||
3 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | reservoir | 2-propanol | 20 (%) | |
6 | 1 | reservoir | PEG4000 | 20 (%) | |
7 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
8 | 1 | drop | spermine | 10 (mM) |