1LZA

DISSECTION OF PROTEIN-CARBOHYDRATE INTERACTIONS IN MUTANT HEN EGG-WHITE LYSOZYME COMPLEXES AND THEIR HYDROLYTIC ACTIVITY

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	79.180, 79.180, 37.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.600
R-factor	0.161
RMSD bond length	0.014
RMSD bond angle	0.035
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000
High resolution limit [Å]	1.500 *
Rmerge	0.064 *
Total number of observations	61953 *
Number of reflections	14960 *
Completeness [%]	75.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.7 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium chloride	3.3 (%(w/v))
3	1	drop	sodium acetate	20 (mM)
4	1	reservoir	sodium chloride	5 (%)
5	1	reservoir	sodium acetate	20 (mM)