1LZ9
ANOMALOUS SIGNAL OF SOLVENT BROMINES USED FOR PHASING OF LYSOZYME
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9188, 0.9195, 0.9198, 0.9201 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.600, 78.600, 37.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8lyz |
| RMSD bond length | 0.032 |
| RMSD bond angle | 0.053 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.035 | 0.182 |
| Number of reflections | 13429 | |
| <I/σ(I)> | 50.2 | 10 |
| Completeness [%] | 99.0 | 80 |
| Redundancy | 8.5 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | CRYSTALLIZED IN HANGING DROPS. 15 MG/ML PROTEIN SOLUTION IN 100 MM SODIUM ACETATE PH 4.6 AND 1 M SODIUM BROMIDE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 0.5 (M) | ||
| 3 | 1 | drop | sodium acetate | 0.1 (M) | |
| 4 | 1 | reservoir | 1 (M) | ||
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
