1LYV
High-resolution structure of the catalytically inactive yersinia tyrosine phosphatase C403A mutant in complex with phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.456, 49.331, 97.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.363 |
| R-factor | 0.129 |
| R-free | 0.19000 |
| Structure solution method | direct replacement |
| Starting model (for MR) | 1hy5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.660 | 1.500 |
| High resolution limit [Å] | 1.363 | 1.360 |
| Rmerge | 0.050 | 0.184 |
| Number of reflections | 55811 | |
| <I/σ(I)> | 50.7 | 4.3 |
| Completeness [%] | 96.5 | 94.8 |
| Redundancy | 2.35 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 18% PEG8000, 200 mM NaCl, TRIS, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
