1LYS
X-RAY STRUCTURE OF A MONOCLINIC FORM OF HEN EGG-WHITE LYSOZYME CRYSTALLIZED AT 313K. COMPARISON OF TWO INDEPENDENT MOLECULES
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.230, 63.660, 59.120 |
| Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.720 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.000 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.720 * |
| Rmerge | 0.061 * |
| Number of reflections | 18391 * |
| Completeness [%] | 87.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.6 * | 313 * | pH was adjusted to 7.6 with 0.1 M HCl * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 1.0 g in 10ml aqueous solution | |
| 2 | 1 | 1 | 10 (%) | ||
| 3 | 1 | 1 | 1-propanol | 5 (%) |
