1LY2
Crystal structure of unliganded human CD21 SCR1-SCR2 (Complement receptor type 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.600, 33.270, 42.110 |
| Unit cell angles | 91.51, 111.44, 117.81 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.179 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Crystal structure of liganded CD21 (complex with C3d) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.340 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.105 * | 0.100 * |
| Total number of observations | 42404 * | |
| Number of reflections | 11265 | |
| Completeness [%] | 88.4 | 55.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298K, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 24 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 30 (%) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
