1LY1
Structure and Mechanism of T4 Polynucleotide Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9790 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 65.300, 91.800, 67.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.000 |
Rwork | 0.210 |
R-free | 0.23100 |
Structure solution method | SIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.051 | 0.223 |
Total number of observations | 36111 * | |
Number of reflections | 12892 | |
<I/σ(I)> | 15.3 | 4.3 |
Completeness [%] | 89.2 | 77.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 22 * | 0.1M NaOAc 4.6, 5mM DTT, 16% PEG2000MME, 0.4M AMSO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
3 | 1 | drop | 0.2 (M) | ||
4 | 1 | drop | imidazole | 0.3 (M) | |
5 | 1 | drop | glycerol | 10 (%) | |
6 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
7 | 1 | reservoir | dithiothreitol | 5 (mM) | |
8 | 1 | reservoir | PEG2000 MME | 16 (%) | |
9 | 1 | reservoir | ammonium sulfate | 0.4 (M) |