1LXZ

Structure of thaumatin crystallized in the presence of glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-12-17
Detector	MARRESEARCH
Wavelength(s)	0.9474
Spacegroup name	P 41 21 2
Unit cell lengths	57.801, 57.801, 149.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.250
R-factor	0.179
Rwork	0.178
R-free	0.19500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1thw
RMSD bond length	0.011
RMSD bond angle	1.581
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.290
High resolution limit [Å]	1.250	1.250
Number of reflections	69080
Completeness [%]	96.8	96.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	glycerol, tartrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K