1LXY
Crystal Structure of Arginine Deiminase covalently linked with L-citrulline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-06-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.1000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.240, 76.370, 82.900 |
Unit cell angles | 90.00, 109.74, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.000 |
R-factor | 0.1861 |
Rwork | 0.186 |
R-free | 0.22700 |
Structure solution method | MIR |
RMSD bond length | 0.009 |
RMSD bond angle | 23.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.083 | 0.220 * |
Number of reflections | 59662 | |
<I/σ(I)> | 11.2 | 4.6 |
Completeness [%] | 97.1 | 90.4 |
Redundancy | 3.37 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | PEG 6000, Potassium phosphate, L-citrulline, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 282K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | PEG 6000, Potassium phosphate, L-citrulline, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 282K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 0.01 (M) | pH7.0 |
3 | 1 | reservoir | PEG6000 | 18 (%) | |
4 | 1 | reservoir | potassium phosphate | 0.1 (M) | pH6.5 |