1LXN

X-RAY STRUCTURE OF MTH1187 NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET TT272

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9791
Spacegroup name	P 41 3 2
Unit cell lengths	175.365, 175.365, 175.365
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.300
Rwork	0.216
R-free	0.24500
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	23.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.058 *
Total number of observations	425012 *
Number of reflections	40380
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5 *	4 *
1	Vapor diffusion *	8.5 *	4 *
1	Vapor diffusion *	8.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)
2	1	drop		500 (mM)
3	1	drop	protein	20 (mg/ml)
4	1	reservoir	sodium acetate	0.1 (M)	pH4.6
5	1	reservoir	ammonium sulfate	2 (M)
6	1	reservoir	glycerol	14 (%)