1LXM
Crystal Structure of Streptococcus agalactiae Hyaluronate Lyase Complexed with Hexasaccharide Unit of Hyaluronan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2000-12-21 |
Detector | OXFORD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.108, 155.043, 238.136 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f1s |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.272 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.144 * | 0.921 * |
Total number of observations | 233927 * | |
Number of reflections | 45372 | |
Completeness [%] | 93.7 | 71.4 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | Jedrzejas, M.J., (2000) Acta Crystallogr., Sect.D, 56, 460. * |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | Jedrzejas, M.J., (2000) Acta Crystallogr., Sect.D, 56, 460. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | cacodylate | 100 (mM) | pH6.0 |
2 | 1 | reservoir | KSCN | 30 (mM) | |
3 | 1 | reservoir | PEG5000 MME | 19 (%) | |
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | drop | HEPES | 10 (mM) | |
6 | 1 | drop | EDTA | 2 (mM) | pH8.0 |