1LXJ
X-RAY STRUCTURE OF YBL001c NORTHEAST STRUCTURAL GENOMICS (NESG) CONSORTIUM TARGET YTYst72
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97895 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 67.380, 67.380, 133.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.800 |
| Rwork | 0.211 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.096 * | |
| Total number of observations | 141589 * | |
| Number of reflections | 12726 | |
| Completeness [%] | 86.8 | 85.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 * | 4 * | Ammonium Sulfate, 2-METHYL-2,4-PENTANEDIOL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | |
| 2 | 1 | drop | 500 (mM) | ||
| 3 | 1 | drop | protein | 20 (mg/ml) | |
| 4 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |
| 5 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 6 | 1 | reservoir | MPD | 12 (%) |
