1LXI
Refinement of BMP7 crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 101.294, 101.294, 41.812 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 * - 2.000 |
| R-factor | 0.22873 |
| Rwork | 0.228 |
| R-free | 0.24400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bmp |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.462 |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 * | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 * | 0.333 * |
| Total number of observations | 67831 * | |
| Number of reflections | 15770 | |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 93.3 * | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 23 * | 20% MPD, 0.1M Sodium Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 20 (%) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.5 |
