1LX7

Structure of E. coli uridine phosphorylase at 2.0A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-15
Detector	MARRESEARCH
Wavelength(s)	0.9794
Spacegroup name	H 3
Unit cell lengths	151.375, 151.375, 48.151
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.000
R-factor	0.182 *
Rwork	0.182
R-free	0.21500
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.038 *	0.080 *
Total number of observations	747216 *
Number of reflections	49742 *
Completeness [%]	89.2 *	78.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	291	0.1M Mes, 10% PEG 4K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	pH8.
3	1	drop		50 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	PEG4000	10 (%)
6	1	reservoir	MES	0.1 (M)	pH6.5
7	1	reservoir	glycerol	5 (%)