1LWX
AZT DIPHOSPHATE BINDING TO NUCLEOSIDE DIPHOSPHATE KINASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 71.590, 71.590, 152.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.300 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.28000 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.510 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 * |
| Rmerge | 0.063 |
| Total number of observations | 134673 * |
| Number of reflections | 19887 |
| Completeness [%] | 86.0 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 25 (mM) | ||
| 5 | 1 | drop | 0.5 (mM) | ||
| 6 | 1 | drop | AZT-DP | 10 (mM) | |
| 7 | 1 | reservoir | PEG550 | 32 (%) |
