1LW7
NADR PROTEIN FROM HAEMOPHILUS INFLUENZAE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9840 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 106.871, 106.871, 174.945 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| Rwork | 0.236 |
| R-free | 0.29800 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.560 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.041 * | 0.409 |
| Total number of observations | 183179 * | |
| Number of reflections | 13327 | |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 98.0 | 97.3 |
| Redundancy | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 20 * | 0.8M Ammonium sulfate, 0.1M MES pH 5.6, Protein Conc. 10 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 100 (mM) | pH7.2 |
| 3 | 1 | drop | 0.3 (M) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
| 6 | 1 | reservoir | ammonium sulfate | 1.0 (M) |
