1LVY
PORCINE ELASTASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW21B |
| Synchrotron site | LURE |
| Beamline | DW21B |
| Temperature [K] | 295 |
| Detector technology | 'BLACK BOX' PROTOTYPE IMAGE PLATE |
| Collection date | 1995-07-14 |
| Detector | J.HENDRIX,A.LENTFER |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.637, 57.973, 75.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.870 |
| R-factor | 0.185 |
| Rwork | 0.187 |
| R-free | 0.22900 |
| Structure solution method | SIRAS |
| Starting model (for MR) | 3est |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.053 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA ((CCP4)) |
| Phasing software | SHARP |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.022 | 0.050 |
| Total number of observations | 74579 * | |
| Number of reflections | 17752 | |
| <I/σ(I)> | 121 | 63.9 |
| Completeness [%] | 92.9 | 78.6 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5.5 | 291 * | CRYSTALLIZED IN NA2SO4 SOLUTION, pH 5.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | acetate | 0.05 (M) |
