1LVH

The Structure of Phosphorylated beta-phosphoglucomutase from Lactoccocus lactis to 2.3 angstrom resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	93
Detector technology	CCD
Collection date	2001-03-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792369, 0.9790073, 0.96112713
Spacegroup name	P 21 21 21
Unit cell lengths	53.667, 92.776, 111.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.890 - 2.300
R-factor	0.2477
Rwork	0.243
R-free	0.28000 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.260 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.081	0.170 *
Total number of observations	420727 *
Number of reflections	23862 *
<I/σ(I)>	12.3	3.5
Completeness [%]	91.0	60.8
Redundancy	16.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	291	Lahiri, S.D., (2002) Acta Crystallogr., Sect.D, 58, 324. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium fluoride	0.2 (M)
2	1	reservoir	PEG3350	20 (%(w/v))	pH6.5
3	1	drop	protein	15 (mg/ml)