1LVG

Crystal structure of mouse guanylate kinase in complex with GMP and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	67.241, 67.241, 108.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.188
R-free	0.23000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ex7
RMSD bond length	0.012
RMSD bond angle	1.538
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.100 *
Rmerge	0.121 *	0.370 *
Total number of observations	105017 *
Number of reflections	14942 *
Completeness [%]	98.4 *	99.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *		protein solution: 10 mg/ml mGMPK, 2mM GMP, 2mM ADP and 5mM MgCl2 reservoir solution: 38-46% PEG 4000, 0.1M Na-citrate pH 5.6, 0.1-0.2 M ammonium acetate

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	GMP	2 (mM)
3	1	drop	ADP	2 (mM)
4	1	drop		5 (mM)
5	1	reservoir	PEG4000	38-46 (%(w/v))
6	1	reservoir	sodium citrate	0.1 (M)	pH5.6
7	1	reservoir	ammonium acetate	0.1-0.2 (M)