1LVF
syntaxin 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-01 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.600, 54.380, 94.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.550 - 2.100 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.20600 * |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.873 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 2.190 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.099 * | 0.345 * |
Number of reflections | 11792 | |
Completeness [%] | 99.6 * | 96.7 |
Redundancy | 4.1 * | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 | 21 * | PEG 8000, sodium acetate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 150 (mM) | ||
2 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
3 | 1 | drop | HEPES | 10 (mM) | pH7.4 |
4 | 1 | reservoir | PEG8000 | 30 (%(v/v)) | |
5 | 1 | reservoir | sodium acetate | 400 (mM) | |
6 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) | |
7 | 1 | reservoir | HEPES | 10 (mM) | pH7.4 |