1LVA
Crystal structure of a C-terminal fragment of Moorella thermoacetica elongation factor SelB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.7250, 0.7255, 0.9773, 0.9783, 0.9840, 0.9050 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.841, 67.006, 105.357 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.200 * - 2.120 |
| Rwork | 0.215 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 20.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.068 * | 0.373 * |
| Total number of observations | 138893 * | |
| Number of reflections | 15941 | |
| <I/σ(I)> | 28.2 | 3.1 |
| Completeness [%] | 100.0 | 99.8 * |
| Redundancy | 8.7 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 * | 298 * | Selmer, M., (2002) Acta Crystallogr., Sect.D, 58, 1871. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 12 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.6 |
