1LVA
Crystal structure of a C-terminal fragment of Moorella thermoacetica elongation factor SelB
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-08-21 |
Detector | MARRESEARCH |
Wavelength(s) | 0.7250, 0.7255, 0.9773, 0.9783, 0.9840, 0.9050 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.841, 67.006, 105.357 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.200 * - 2.120 |
Rwork | 0.215 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 20.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.200 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.068 * | 0.373 * |
Total number of observations | 138893 * | |
Number of reflections | 15941 | |
<I/σ(I)> | 28.2 | 3.1 |
Completeness [%] | 100.0 | 99.8 * |
Redundancy | 8.7 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 * | 298 * | Selmer, M., (2002) Acta Crystallogr., Sect.D, 58, 1871. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 16 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 12 (%) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.6 |