1LV7

Crystal Structure of the AAA domain of FtsH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	120
Detector technology	CCD
Collection date	2002-01-26
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 41 21 2
Unit cell lengths	53.152, 53.152, 189.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.500
R-factor	0.15488
Rwork	0.154
R-free	0.17800 *
Structure solution method	MIRAS
RMSD bond length	0.018
RMSD bond angle	1.811 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.1.12)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	1.500 *	1.500 *
Rmerge	0.064 *	0.261 *
Total number of observations	913258 *
Number of reflections	44655 *
Completeness [%]	98.4 *	91.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	292	ammonium sulfate, Tris-HCl, DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH8.5
2	1	drop		50 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	protein	10 (mg/ml)
5	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
6	1	reservoir	ammonium sulfate	1.5 (M)