1LV2

Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-05
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	152.708, 152.708, 93.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.700
R-factor	0.247
Rwork	0.245
R-free	0.26800 *
Structure solution method	SIRAS
Starting model (for MR)	Built by fitting pieces of secondary structure into density.
RMSD bond length	0.009 *
RMSD bond angle	1.675 *
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (2000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.048	0.320 *
Total number of observations	1308098 *
Number of reflections	18866 *
<I/σ(I)>	36.3	3.5
Completeness [%]	98.4 *	93.7 *
Redundancy	15.8	15.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	294	ammonium phosphate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 21K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	Tris	10 (mM)	pH8.0
3	1	drop	EDTA	0.1 (mM)
4	1	drop		150 (mM)
5	1	drop	dithiothreitol	10 (mM)
6	1	drop	propane-diol	5 (%)
7	1	reservoir	ammonium dihydrogen phosphate/diammonium hydrogen phosphate	0.75 (M)	pH5.0
8	1	reservoir	dithiothreitol	10 (mM)