1LV1

Crystal Structure Analysis of the non-active site mutant of tethered HIV-1 protease to 2.1A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200HB
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2001-01-01
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	63.060, 63.060, 83.420
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 * - 2.100
R-factor	0.1935 *
Rwork	0.194
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	26.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.071
Total number of observations	51200 *
Number of reflections	10245 *
<I/σ(I)>	10.1
Completeness [%]	93.1	85 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	295	5% saturated ammonium sulfate, 200mM sodium phosphate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	sodium acetate	50 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	reservoir	ammonium sulfate	5 (%sat)
5	1	reservoir	sodium phosphate	200 (mM)
6	1	reservoir	sodium citrate	100 (mM)	pH6.2