1LUL
DB58, A LEGUME LECTIN FROM DOLICHOS BIFLORUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.390, 130.950, 138.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.300 |
| R-factor | 0.227 * |
| Rwork | 0.226 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.580 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.135 | 0.279 |
| Total number of observations | 47794 * | |
| Number of reflections | 22745 | |
| <I/σ(I)> | 10.2 | 5.5 |
| Completeness [%] | 80.6 | 68 |
| Redundancy | 2.07 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 100 MM NA-CITRATE PH 5.6 10 % (W/V) PEG 6000 0.3 M NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-7 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 12 (%(w/v)) | |
| 4 | 1 | reservoir | 0.2-0.4 (M) |
