1LUL

DB58, A LEGUME LECTIN FROM DOLICHOS BIFLORUS

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-03
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	101.390, 130.950, 138.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.300
R-factor	0.227 *
Rwork	0.226
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bjq
RMSD bond length	0.011
RMSD bond angle	1.580 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.135	0.279
Total number of observations	47794 *
Number of reflections	22745
<I/σ(I)>	10.2	5.5
Completeness [%]	80.6	68
Redundancy	2.07	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6		100 MM NA-CITRATE PH 5.6 10 % (W/V) PEG 6000 0.3 M NACL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-7 (mg/ml)
2	1	reservoir	sodium citrate	100 (mM)
3	1	reservoir	PEG6000	12 (%(w/v))
4	1	reservoir		0.2-0.4 (M)