1LUF

Crystal Structure of the MuSK Tyrosine Kinase: Insights into Receptor Autoregulation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-29
Detector	CUSTOM-MADE
Wavelength(s)	0.979
Spacegroup name	I 4
Unit cell lengths	146.939, 146.939, 39.017
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 2.050
R-factor	0.231
Rwork	0.231
R-free	0.25200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1IRK
RMSD bond length	0.007 *
RMSD bond angle	1.300 *
Data reduction software	MADNESS
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.056 *	0.319 *
Total number of observations	216370 *
Number of reflections	28438 *
Completeness [%]	99.7	99.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	20 *	ammonium sulfate, HEPES, glycerol, TCEP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	reservoir	ammonium sulfate	0.75 (M)
3	1	reservoir	HEPES	0.1 (M)	pH7.0
4	1	reservoir	glycerol	2 (%)
5	1	reservoir	TCEP	1.3 (mM)
6	1	reservoir	sodium thiocyanate	0.1 (M)