1LUC
BACTERIAL LUCIFERASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.500, 59.000, 76.500 |
| Unit cell angles | 90.00, 93.86, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.182 * |
| RMSD bond length | 0.015 |
| RMSD bond angle | 15.423 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.041 |
| Total number of observations | 297839 * |
| Number of reflections | 105158 * |
| Completeness [%] | 99.0 * |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ME-PEG5000 | 17 (%) | |
| 2 | 1 | 1 | 250 (mM) | ||
| 3 | 1 | 1 | MES | 100 (mM) | |
| 4 | 1 | 1 | protein | 7.5 (mg/ml) |
