1LU9
Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.130, 85.240, 92.370 |
| Unit cell angles | 90.00, 113.81, 90.00 |
Refinement procedure
| Resolution | 29.890 - 1.900 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.22900 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 23.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.220 |
| Number of reflections | 69818 | |
| <I/σ(I)> | 20.3 | 3 |
| Completeness [%] | 93.8 | 84 |
| Redundancy | 3.0 * | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | Hepes, PEG1500, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | HEPES | 100 (mM) | pH7.5 |
| 3 | 1 | reservoir | PEG1500 | 20 (%) | |
| 4 | 1 | reservoir | Tris | 20 (mM) | pH8.5 |
