1LTT
LACTOSE BINDING TO HEAT-LABILE ENTEROTOXIN REVEALED BY X-RAY CRYSTALLOGRAPHY
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 119.800, 101.200, 64.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.000 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 * |
| Rmerge | 0.064 * |
| Completeness [%] | 91.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.5 * | three-layer liquid-liquid diffusion, taken from Pronk, S.E. et al (1985). J. Biol. Chem., 260, 13580-13584. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 10 | 1 | 3 | 0.3 (M) | ||
| 11 | 1 | 3 | PEG6000 | 15 (%) | |
| 2 | 1 | 1 | EDTA | 1 (mM) | |
| 3 | 1 | 1 | 3 (mM) | ||
| 4 | 1 | 1 | KF | 0.3 (M) | |
| 5 | 1 | 2 | lactose | 25 (mM) | |
| 7 | 1 | 1 | precipitant | 10 (mg/ml) | |
| 8 | 1 | 3 | Tris-HCl | 50 (mM) | |
| 9 | 1 | 3 | EDTA | 3 (mM) |
