1LTS

REFINED STRUCTURE OF E. COLI HEAT LABILE ENTEROTOXIN, A CLOSE RELATIVE OF CHOLERA TOXIN

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	119.200, 98.200, 64.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.950
R-factor	0.182
RMSD bond length	0.150 *
RMSD bond angle	3.000 *
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.950 *
Rmerge	0.088 *
Total number of observations	230816 *
Number of reflections	53762 *
Completeness [%]	94.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *			two-layer method, taken from Pronk, S.E. et al (1985). J. Biol. Chem., 260, 13580-13584. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	50 (mM)
2	1	1	EDTA	1 (mM)
3	1	1		3 (mM)
4	1	1	KF	0.3 (M)
5	1	1	precipitant	10 (mg/ml)
6	1	2	Tris-HCl	50 (mM)
7	1	2	EDTA	1 (mM)
8	1	2		3 (mM)
9	1	2	PEG6000	15 (%)