1LTG

THE ARG7LYS MUTANT OF HEAT-LABILE ENTEROTOXIN EXHIBITS GREAT FLEXIBILITY OF ACTIVE SITE LOOP 47-56 OF THE A SUBUNIT

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-10-31
Detector	SIEMENS-NICOLET X100
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	119.700, 98.500, 65.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.178
Rwork	0.178
RMSD bond length	0.013
RMSD bond angle	3.000
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.073
Total number of observations	60917 *
Number of reflections	22826 *
Completeness [%]	75.0 *	54 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	4.2 (mg/ml)
2	1	2	NAD	18 (mM)
3	1	2	guanyltyramine	0.8 (mM)
4	1	2		210 (mM)
5	1	2	PEG	6.7 (%)