1LTD

THE 2.6 ANGSTROMS REFINED STRUCTURE OF THE ESCHERICHIA COLI RECOMBINANT SACCHAROMYCES CEREVISIAE FLAVOCYTOCHROME B2-SULPHITE COMPLEX

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	164.500, 164.500, 114.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.600
R-factor	0.173
Rwork	0.173
RMSD bond length	0.006
RMSD bond angle	3.370
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.600 *	2.600 *
Rmerge	0.147 *
Total number of observations	452385 *
Number of reflections	44282 *
Completeness [%]	81.0 *	55 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		5 (mM)
2	1	drop	enzyme	25 (mg/ml)
3	1	reservoir	PEG4000	12 (%)
4	1	reservoir	MES	50 (mM)